@ezromeo
If @mkst is correct in thinking that you are talking about the girls bunching up in the small size multi-girl desktop mode rather than in the scene mode then the reason for this is the way in which iStripper spreads the clips across the width of the screen. The following is an approximation to how it is done. For simplicity I will restrict myself to the case where there are only simple standing clips including pole dance clips.
Firstly, although each clip looks to be quite narrow they are, when played at 100% size, effectively approximately the same size and shape as your screen and during their performance the girls are free to wander all over this area.
When the small size multi-girl desktop mode is used the program, by default, will position smaller versions of these screen shaped areas evenly spaced across the screen but in such a way that they are all fully on the screen.
If you have configured to play only a small number of clips simultaneously or if the size you have specified for those clips is low then the program can position them such that they do not overlap. But if too many clips are to be displayed or a large size has been specified then the areas used for each clip will overlap and the distance between their centres will be reduced in order to keep them all completely on the screen. It is this that cause the bunching effect that you see (and that many of us, myself included, dislike).
Think what would happen if multiple clips were all displayed at 1005 size. They would all be the same size as the screen and in order for any of them to fully fit on the screen they would have to be displayed at the same place.
I am sure that the above is wrong in detail as the program seems to reposition clips only when something "untoward" happens (such as a girl walking off the edge of the screen) rather than simply using pre-calculated positions but the end result is much the same.
The solution is simple - go to the setting page and reduce the number of clips to be played, or their size or both.
Note, the program keeps track of where card centres have been have repositioned and will continue to use these memorised positions until you reset them. You can do this by ***** the each girl to a new position until you are satisfied (remember to ***** them slightly up the screen so that they drop down to the task bar or bottom of the screen rather than dropping off the bottom). A quicker way to reset the positions may be to exit the program 9using the Quit menu entry, closing the window does not quit the program), delete the file holding them (I think this is called entrances.dat) and restarting iStripper.